STORM (Stochastic Optical Reconstruction Microscopy) is an advanced imaging technique that enables visualization of cellular structures beyond the diffraction limit, achieving near-molecular scale imaging. By selectively activating and deactivating fluorescent molecules, STORM captures multiple images over time, which are computationally reconstructed into a high-resolution image. This method is vital for studying dynamic processes in live cells, offering insights into molecular interactions and localization.

CLEM (Light Electron Microscopy) is an imaging technique that combines light and electron microscopy data to enhance understanding of cellular structures and processes. Initially, samples are imaged with light microscopy for contextual information, followed by electron microscopy to capture detailed ultrastructural data. This combination allows us to visualize microscopic features and correlate them with physiological observations, providing complementary data for a comprehensive biological analysis.

STED microscopy (Stimulated Emission Depletion) is an advanced imaging technique that exceeds the diffraction limit of traditional light microscopy, enabling us to visualize biological structures with nanometer resolution. It utilizes two lasers: one excites fluorescent tags on the specimen, while the other depletes fluorescence outside a small region of interest, creating a "doughnut" shape. By scanning this focused area, we can generate high-resolution images.

STED microscopy offers enhanced spatial resolution of 20 to 50 nanometers, facilitating detailed studies of protein complexes and cellular membranes, particularly in live cells.